Introduction to RNA Sequencing Bioinformatics

A Tufts University Research Technology Workshop

Description:

This tutorial will cover the basics of bioinformatics for RNA sequencing analysis using command line tools and R on the Tufts High Performance Compute Cluster (HPC). The analysis pipeline includes quality control, read alignment, feature quantification, differential expression and pathway analysis. There was a 1-hour introductory session, after which students will follow this self-guided online material. The material is designed to run on Tufts High Performance Compute (HPC) Cluster. If you don’t have access to Tufts HPC, you will need to install all required modules on your own computer.

Goals

Prerequisites

Basic understanding of computational skills

Materials Needed

If you are a Tufts member and have access to Tufts HPC:

If you don’t have access to Tufts HPC:

All scripts you will be using:

1-hr introduction session slides:

Schedule

Acknowledgement

Much of this workshop was adapted from Bioinformatics @ Tufts and the HBC Training DGE workshop with the help of Dr. Rebecca Batorsky and Dr. Albert Tai at Tufts University.